Genetic variability among archival cultures of Salmonella typhimurium

By Kelly Edwards, Irina Linetsky, Christopher Hueser, and Abraham Eisenstark. (published in FEMS Microbiology Letters, 2001)

Table 1. Salmonella typhimurium strains used for this study*.

Lab #

Allele Inoc Date cfu on LB cfu on Min.
1568 thyA36 9-64 106×105 91×105
1594a his2555 4-58 21×104 38×103
1596 his-2555 4-58 128×104 218×103
1598 his141 2-58 122×103 0
1599 his142 6-54 41×103 230
1600 his143 6-54 142×103 151×103
1601 his144 6-54 3×104 0
1602b X3000 10-98 76×103 0
1605 purF145 1-61 21×103 2×103
1608 pur4391 1-61 187×105 121×105
1609 purD35 1-61 81×105 2×103
1613 thyA162 7-64 86×105 73×105
1614 his4384 8-54 286×103 302×103
1615 his2543 1-58 26×105 0
1616 nic6 1-58 218×103 196×103
1617 his4385 8-64 202×103 183×103
1619 aro63 8-64 86×103 0
1626 thyA173 8-54 65×102 0
1632 his2555 8-54 186×104 137

 

Lab #

Allele Inoc Date cfu on LB cfu on Min.
1635 his142 6-54

210×104

193×103
1636 his142 6-54 132×104 0
1637 his142 6-54 46×102 0
1638 his113 6-54 121×103 0
1639 his142 6-54 126×104 102×104
1640 his142 6-54 26×102 0
1648 thyA1566 8-58 234×103 127×103
1652 his2526 8-58 66×103 0
1653 aro32 8-58 22×103 0
1654 aro32 8-58 87×103 0
1656 thyA68 5-64 18×103 0
1657 his-2122 4-58 72×103 68×103
1658 his-2122 4-58 102×103 93
1659 his2122 4-58 138×103 127×103
1661 trp-138 6-60 302×103 287×103
1667 his2550 4-58 102×102 0
1670 his2550 4-67 28×103 0
1671 his2550 4-58 74×102 210
1672 his2550 4-58 81×102 460

 

Lab #

Allele Inoc Date cfu on LB cfu on Min.
1674 his-2550 8-58

82×104

73×104
1681 thyA270 8-64 312×102 11×103
1684 thyA273 8-64 1000 0
1685 thyA269 8-64 2050 0
1686 thyA273 8-64 12×103 0
1687 thyA273 8-64 52×104 31×104
1688 thyA272 8-64 2120 0
1690 thyA271 8-64 123×105 0
1692 thyA275 8-64 1530 0
1693 thyA274 8-64 152×103 147×103
1695 thyA272 8-64 140×103 129×103
1699 thyA314 8-64 136×105 128×105
1700 thyA314 8-64 13×104 9×104
1701 thyA314 8-64 42×103 47×103
1702 thyA315 8-64 106×103 112×103
1703 thyA314 8-64 110 0
1704 thyA316 8-64 82×103 37×103
1706 thyA316 8-64 212×104 102×103
1732 thyA323 8-64 42×102 71×102

 

Lab # Allele Inoc Date cfu on LB cfu on Min.
1734 thyA323 8-64 123×102 131×102
1737 thyA324 8-64 123×102 108×102
1738 thyA324 8-64 11×103 14×103
1745 thyA329 8-64 30×102 0
1747 his-1 8-64 20×104 0
1748 his-3 8-64 74×104 8
1918 aro-57 5-60 16×103 19×103
1922 thyA158 1-65 64×104 0
1923 his-2526 4-58 216×104 0
1927 aro-20 8-57 520×103 0
1929 thyA68 1-65 231×104 211×104
1931 aro-65 1-62 206×104 146×104
1932 his-2555 4-58 315×104 31×104
1933 his-2555 4-58 181×104 188×104
1934 aro-43 8-59 83×105 74×105
1935 thyA2431 3-65 206×103 20×105
1939 thyA2422 3-65 108×105 19×103
1940 pur-11 N/Ac 126×103 30×103
1942 pur-8 N/A 392×103 160×103
1944 purE150 10-55 288×103 42×103

 

 

* Starting in the late 1940âs, Miloslav Demerec and associates at Cold Spring Harbor Laboratory and Brookhaven National Laboratory stocked an extensive collection of auxotrophic mutants for the purpose of mapping the S. typhimurium LT2 chromosome. Over 10,000 of these original sealed agar stab cultures (0.5 ml/agar stab vial) are now curated in our laboratory at the Cancer Research Center. Information on alleles is from labels on vials or from original notebooks.

a. Some allele numbers are duplicated in the sample set. When archival vials were initially opened and plated, some polymorphism in colony shape and size was observed. These individual colonies were selected and re-isolated after which they were given individual laboratory identification numbers. Additionally, some cultures gave rise to new colony types after plating on specific media. These subcultures were also given unique numbers while maintaining the original allelic identification number.

b. Strain 1602 is the reference strain X3000, a gift from Cheryl Nickerson.

c. N/A Date of isolation was not found in notebook.

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